SnapGene $5 gaattccggatgagcattcatcaggcgggcaagaatgtgaataaaggccggataaaacttgtgcttatttttctttacggtctttaaaaaggccgtaatatccagctgaacggtctggttataggtacattgagcaactgactgaaatgcctcaaaatgttctttacgatgccattgggatatatcaacggtggtatatccagtgatttttttctccattttagcttccttagctcctgaaaatctcgataactcaaaaaatacgcccggtagtgatcttatttcattatggtgaaagttggaacctcttacgtgccgatcaacgtctcattttcgccaaaagttggcccagggcttcccggtatcaacagggacaccaggatttatttattctgcgaagtgatcttccgtcacaggtatttattcggcgcaaagtgcgtcgggtgatgctgccaacttactgatttagtgtatgatggtgtttttgaggtgctccagtggcttctgtttctatcagctgtccctcctgttcagctactgacggggtggtgcgtaacggcaaaagcaccgccggacatcagcgctagcggagtgtatactggcttactatgttggcactgatgagggtgtcagtgaagtgcttcatgtggcaggagaaaaaaggctgcaccggtgcgtcagcagaatatgtgatacaggatatattccgcttcctcgctcactgactcgctacgctcggtcgttcgactgcggcgagcggaaatggcttacgaacggggcggagatttcctggaagatgccaggaagatacttaacagggaagtgagagggccgcggcaaagccgtttttccataggctccgcccccctgacaagcatcacgaaatctgacgctcaaatcagtggtggcgaaacccgacaggactataaagataccaggcgtttccccctggcggctccctcgtgcgctctcctgttcctgcctttcggtttaccggtgtcattccgctgttatggccgcgtttgtctcattccacgcctgacactcagttccgggtaggcagttcgctccaagctggactgtatgcacgaaccccccgttcagtccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggaaagacatgcaaaagcaccactggcagcagccactggtaattgatttagaggagttagtcttgaagtcatgcgccggttaaggctaaactgaaaggacaagttttggtgactgcgctcctccaagccagttacctcggttcaaagagttggtagctcagagaaccttcgaaaaaccgccctgcaaggcggttttttcgttttcagagcaagagattacgcgcagaccaaaacgatctcaagaagatcatcttattaatcagataaaatatttctagatttcagtgcaatttatctcttcaaatgtagcacctgaagtcagccccatacgatataagttgtaattctcatgtttgacagcttatcatcgataagctttaatgcggtagtttatcacagttaaattgctaacgcagtcaggcaccgtgtatgaaatctaacaatgcgctcatcgtcatcctcggcaccgtcaccctggatgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgttctcgacccccgtcgcgccgtaactcaggctagcgcaggttttgagcgctggcgctttacccactgcgccctgccagagctgaattttagcgacatcacgctggaaaccaccttcctgaatcggcagctacaggctccgctgctgatcagctccatgaccggcggcgttgagcgctcgcgccatatcaaccgccacctcgccgaggcggcgcaggtgctaaaaattgcgatgggggtgggctcccagcgcgtcgccattgagagcgacgcgggcttagggctggataaaaccctgcggcagctggctccggacgtgccgctgctggcgaacctcggcgcggcgcagctgaccggcagaaaaggtattgattacgcccgacgggccgtggagatgatcgaggcggatgcgctgattgtgcacctaaacccgctgcaggaggcgctacagcccggcggcgatcgcgactggcgcggacggctggcggctattgaaactctggtccgcgagctgcccgttccgctggtggtgaaagaggtgggagccggtatctcccgaaccgtggccgggcagctgatcgatgccggcgttaccgtgattgacgtcgcgggcgcgggcggcaccagctgggccgccgttgaaggcgagcgggcggccaccgagcagcagcgcagcgtggccaacgtctttgccgactgggggatccccaccgctgaggcgctggttgacattgccgaggcctggccgcagatgccccttattgcctcgggcgggattaaaaacggcgtcgaccagccgcgctacgttgccgaggctaatctggtgatcacccacggcggtctcaataccgtactggatgcgctggctgccgcgacgccggtgctggcggtgccactctctttcgaccagcccgccgtggctgcccggctggtctataacgggctgggtcgccgggtatcgcgctttgccagacagcagacgctggcggatgagattgcccaactgctgggggatgagacgctgcatcagcgtctggcgacggcccgccagcagcttaacgacgccgggggcacgccccgtgcggcgaccctgattgaacaggccatagcagggagtgagagcgtatcgtgagggatctgattttagtcggcggcggcctggccaacgggctgatcgcctggcgtctgcgccagcgctacccgcagcttaacctgctgctgatcgaggccggggagcagcccggcgggaaccatacctggtcattccatgaagacgatctgactcccgggcagcacgcctggctggccccgctggtggcccacgcctggccgggctatgaggtgcagtttcccgatcttcgccgtcgcctcgcgcgcggctactactccattacctcagagcgctttgccgaggccctgcatcaggcgctgggggagaacatctggctaaactgttcggtgagcgaggtgttacccaatagcgtgcgccttgccaacggtgaggcgctgcttgccggagcggtgattgacggacgcggcgtgaccgccagttcggcgatgcaaaccggctatcagctctttcttggtcagcagtggcggctgacacagccccacggcctgaccgtaccgatcctgatggatgccacggtggcgcagcagcagggctatcgctttgtctacacgctgccgctctccgccgacacgctgctgatcgaggatacgcgctacgccaatgtcccgcagcgtgatgataatgccctacgccagacggttaccgactatgctcacagcaaagggtggcagctggcccagcttgaacgcgaggagaccggctgtctgccgattaccctggcgggtgacatccaggctctgtgggccgatgcgccgggcgtgccgcgctcgggaatgcgggctgggctatttcaccctaccactggctattcgctgccgctggcggtggcccttgccgacgcgattgccgacagcccgcggctgggcagcgttccgctctatcagctcacccggcagtttgccgaacgccactggcgcaggcagggattcttccgcctgctgaaccggatgcttttcctggccgggcgcgaggagaaccgctggcgggtgatgcagcgcttttatgggctgccggagcccaccgtagagcgcttttacgccggtcggctctctctctttgataaggcccgcattttgacgggcaagccaccggttccgctgggcgaagcctggcgggcggcgctgaaccattttcctgacagacgagataaaggatgaaaaaaaccgttgtgattggcgcaggctttggtggcctggcgctggcgattcgcctgcaggcggcagggatcccaaccgtactgctggagcagcgggacaagcccggcggtcgggcctacgtctggcatgaccagggctttacctttgacgccgggccgacggtgatcaccgatcctaccgcgcttgaggcgctgttcaccctggccggcaggcgcatggaggattacgtcaggctgctgccggtaaaacccttctaccgactctgctgggagtccgggaagaccctcgactatgctaacgacagcgccgagcttgaggcgcagattacccagttcaacccccgcgacgtcgagggctaccggcgctttctggcttactcccaggcggtattccaggagggatatttgcgcctcggcagcgtgccgttcctctcttttcgcgacatgctgcgcgccgggccgcagctgcttaagctccaggcgtggcagagcgtctaccagtcggtttcgcgctttattgaggatgagcatctgcggcaggccttctcgttccactccctgctggtaggcggcaaccccttcaccacctcgtccatctacaccctgatccacgcccttgagcgggagtggggggtctggttccctgagggcggcaccggggcgctggtgaacggcatggtgaagctgtttaccgatctgggcggggagatcgaactcaacgcccgggtcgaagagctggtggtggccgataaccgcgtaagccaggtccggctggcggatggtcggatctttgacaccgacgccgtagcctcgaacgctgacgtggtgaacacctataaaaagctgctcggccaccatccggtggggcagaagcgggcggcagcgctggagcgcaagagcatgagcaactcgctgtttgtgctctacttcggcctgaaccagcctcattcccagctggcgcaccataccatctgttttggtccccgctaccgggagctgatcgacgagatctttaccggcagcgcgctggcggatgacttctcgctctacctgcactcgccctgcgtgaccgatccctcgctcgcgcctcccggctgcgccagcttctacgtgctggccccggtgccgcatcttggcaacgcgccgctggactgggcgcaggaggggccgaagctgcgcgaccgcatctttgactaccttgaagagcgctatatgcccggcctgcgtagccagctggtgacccagcggatctttaccccggcagacttccacgacacgctggatgcgcatctgggatcggccttctccatcgagccgctgctgacccaaagcgcctggttccgcccgcacaaccgcgacagcgacattgccaacctctacctggtgggcgcaggtactcaccctggggcgggcattcctggcgtagtggcctcggcgaaagccaccgccagcctgatgattgaggatctgcaatgagccaaccgccgctgcttgaccacgccacgcagaccatggccaacggctcgaaaagttttgccaccgctgcgaagctgttcgacccggccacccgccgtagcgtgctgatgctctacacctggtgccgccactgcgatgacgtcattgacgaccagacccacggcttcgccagcgaggccgcggcggaggaggaggccacccagcgcctggcccggctgcgcacgctgaccctggcggcgtttgaaggggccgagatgcaggatccggccttcgctgcctttcaggaggtggcgctgacccacggtattacgccccgcatggcgctcgatcacctcgacggctttgcgatggacgtggctcagacccgctatgtcacctttgaggatacgctgcgctactgctatcacgtggcgggcgtggtgggtctgatgatggccagggtgatgggcgtgcgggatgagcgggtgctggatcgcgcctgcgatctggggctggccttccagctgacgaatatcgcccgggatattattgacgatgcggctattgaccgctgctatctgcccgccgagtggctgcaggatgccgggctgaccccggagaactatgccgcgcgggagaatcgggccgcgctggcgcgggtggcggagcggcttattgatgccgcagagccgtactacatctcctcccaggccgggctacacgatctgccgccgcgctgcgcctgggcgatcgccaccgcccgcagcgtctaccgggagatcggtattaaggtaaaagcggcgggaggcagcgcctgggatcgccgccagcacaccagcaaaggtgaaaaaattgccatgctgatggcggcaccggggcaggttattcgggcgaagacgacgagggtgacgccgcgtccggccggtctttggcagcgtcccgtttaggcgggcggccatgacgttcacgcaggatcgcctgtaggtcggcaggcttgcgggcgtaaataaaaccgaaggagacgcagccctcccggccgcgcaccgcgtggtgcaggcggtgggcgacgtagagccgcttcaggtagccccggcgcgggatcccgcctcgccgtgctgtccggtctcaacctgatccgccagaatcgagccaacgggatcggccagcacgaattcggtatgcggagagtgtttggcaaaccatgcctgcaagccacccagcgtgccgccggagccaacgcctactaccacggcatcaaccctgcctgccagctggtcgaacagctccggtgcggtggtggtggcgtgcgccagcgggttggccgggttagagaactgatcaatatagtaagcacccggcgtctcctctgccaggcggcgggcgtagtcctgatagtactccgggtggccctttgtcacgtcggagcgggtcaggcgcacatcaacacccagcgcacgcaggtggtagatcctctacgccggacgcatcgtggccggcatcaccggcgccacaggtgcggttgctggcgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggcgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctactactgggctgcttcctaatgcaggagtcgcataagggagagcgtcgaccgatgcccttgagagccttcaacccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgtcttctttatcatgcaactcgtaggacaggtgccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcactggtcccgccaccaaacgtttcggcgagaagcaggccattatcgccggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgaggctggatggccttccccattatgattcttctcgcttccggcggcatcgggatgcccgcgttgcaggccatgctgtccaggcaggtagatgacgaccatcagggacagcttcaaggatcgctcgcggctcttaccagcctaacttcgatcactggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggcgccgccctataccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctgaatggaagccggcggcacctcgctaacggattcaccactccaagaattggagccaatcaattcttgcggagaactgtgaatgcgcaaaccaacccttggcagaacatatccatcgcgtccgccatctccagcagccgcacgcggcgcatctcgggcagcgttgggtcctggccacgggtgcgcatgatcgtgctcctgtcgttgaggacccggctaggctggcggggttgccttactggttagcagaatgaatcaccgatacgcgagcgaacgtgaagcgactgctgctgcaaaacgtctgcgacctgagcaacaacatgaatggtcttcggtttccgtgtttcgtaaagtctggaaacgcggaagtcccctacgtgctgctgaagttgcccgcaacagagagtggaaccaaccggtgataccacgatactatgactgagagtcaacgccatgagcggcctcatttcttattctgagttacaacagtccgcaccgctgtccggtagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgtcttctttatcatgcaactcgtaggacaggtgccggcagcgcccaacagtcccccggccacggggcctgccaccatacccacgccgaaacaagcgccctgcaccattatgttccggatctgcatcgcaggatgctgctggctaccctgtggaacacctacatctgtattaacgaagcgctaaccgtttttatcaggctctgggaggcagaataaatgatcatatcgtcaattattacctccacggggagagcctgagcaaactggcctcaggcatttgagaagcacacggtcacactgcttccggtagtcaataaaccggtaaaccagcaatagacataagcggctatttaacgaccctgccctgaaccgacgaccgggtcgaatttgctttcgaatttctgccattcatccgcttattatcacttattcaggcgtagcaccaggcgtttaagggcaccaataactgccttaaaaaaattacgccccgccctgccactcatcgcagtactgttgtaattcattaagcattctgccgacatggaagccatcacagacggcatgatgaacctgaatcgccagcggcatcagcaccttgtcgccttgcgtataatatttgcccatggtgaaaacgggggcgaagaagttgtccatattggccacgtttaaatcaaaactggtgaaactcacccagggattggctgagacgaaaaacatattctcaataaaccctttagggaaataggccaggttttcaccgtaacacgccacatcttgcgaatatatgtgtagaaactgccggaaatcgtcgtggtattcactccagagcgatgaaaacgtttcagtttgctcatggaaaacggtgtaacaagggtgaacactatcccatatcaccagctcaccgtctttcattgccatacg Nk " ) J , 1 H > E L )
4 1 5 - $ 3 & ' f 1 1 i P " % { % / 0 f J : 5 = ? ' * ' 0 $ , ' . , ) $ & 7 + ? & . & . [ & ) 1 .
5 4 G
# ! $ & & " # ! ( ( 6 # " % & $ P 7 l $ 1 % & > 0 \ e C S ' " ' &
? g ; S & % & ( & _ ( Y ; & 0 F # ' 7 ) " ~N O
b * + B =/B6 P | !##) r1P
c q , Cc
O9Uc{C| e!!P#{# p E
M
XU^t Q "$J _< @
#
^6KY
L >>0XLZ0 7 - a v } a"#, L J
d -
^Dd c:V, [ f!! ,"2r D t 2!"|###1 1 {[)Q o
G
ab;K Pd|P } " ! !Q# s<4o n > H " #ql
E N m
7% )ehWf}LU:?BEz- s T ! s #A##$ { h ey N
vvAImu f !!# t =pg }
B{V_ R ^ # N o 6
F
~ YV
, !" :7:cf&MZ]jw a
l
0 n $ r
4LVZ]@Wc ?@ 03i RRYvH'o1 < s * { M w L"X"#> -eC""Me
_{7 S"h$}oY }8 y v ~
~ i!!b"""E `XWksz *
2
A
$ 8
#Z lt 1bM[. L D I '!_!!V#L - Z Y=
" : v {
^
:' OMk&Fm ?Q5=Z ! T b 9 !,##) n:a$K_ t
\ L p E
>_
9dW J < g!
8 Ss
.
wEqC;-d & !!Z#
Mt
~ ( K 4 M@ )
e
H l`
e7Fbb \ y 5 !!# z#3q O,]4:)E u I!!"# # %q). y #D#_#}##### & D p f j m!"&""# k -A!
s 0
c K+0f } 3! ""$1 k\{ * p
f
2E]? Q ~ !"# 2 &H
i
"yoQr |U2 u
Ib5c # R#5 =it' `R ( X A
(/9Gjsc<L * =e}kH * !u!- xp f
z
D3t"nAZ/\ % E ""\# t=ek _
6
Pp=|6o !" 5fJ
+s= R " $ S`3
ZYg*oi k ? !"3 !8 (
8ffigJV@CFr{. t 0!#& $ #x6
F n d
& *X ~ #B#$ Or(O ##' < 8$}m6
^
( M;K t U " .
bM_nk ' g !O"# ^ (fwxJ u !"## iQ9*O
wBv A!"# | z " )Vb= h W! J 9 S " # u
qQ ~
`_ M L>F |2Q~6j U!["2#### &J
A eCF-?
! G
xZ - D O 6 ""# t DS h * ~!!! O p D oA 7
3
p2V 4 < "$ OvZ
W V b ##f J
A;8d'[mx
m
o
5XW[
@`1 `PSSy=S 4 t R | N 7 z !"1 y^d
J
GM; ( Z C H w { ###1 &;6
1 \ b
c&dnvK40HIp= H M M"Y""i DZ[;BHg-Nk1 b T
7 = % ` s
M
^8AXEGA9!j"]AwM(d2 } ( + $ x L!P #]iG+ . 3 c
F g
_
`|T#Qi~ pdH-Z2O~z c !j!" .D6O
8 Axd !!"
jf
,Z%#
9 w W & !!"""% 4f=#N8
C#pCyJ
Z !!R"c""{ h 1W_!$/Dt \ d
+
3
B
i
% R U ' u &
;
>
+1[H`CmpBT/b=gTR 'Xl2>m)$ * 9 M J _ !!$!W#% FoP{ ? 9
O
$];cp/ E (!N!!/ > aYlV
I x
:WUgf m ! "9"0 X{*= ,D ,
js] u5N\$ 0 j `!#$f . [ :9>Iv^ G q |
%
J
_ | )
3
.AM
Ps*INlO@{
i>d[3G v " - ^ x "#J O JV# ;
[
9 g T
v
<|P$*H_n
#l%OR
c 0 .""""-###,$: \w , b R '1G.R[$6_ !* U @ V : !!o""5 Z]1 V !
.
y
j o
;T(TB~Kw)5q
0 """# ^qo0;
< `"O#z#$ z!IF
~` :Jb+m = ! z k M eH| lr #= , Y V!9!djb%L ` u
] # q
&?VkbXQ K ) H ^!h!!K"W"# 9 LR
k_6ac ' !# p/ e e l!! ## %Ts t
xFrD<G _^
e
y
srf.!"[# Nw|5
t" (U% ~} L ## Nu
w ) U !? CIA 0 5
< I [ a
b%m8_/O\Rc|
z 6 K!!"#' 3iN* - b
f
owx b !!!Q"""* g 0+
H
rS\.a<fS=(4# i $' HU
i S;Q{0O#-G4cp ] w !" + X 8
%};G{* _" $ $Qor ^5F # 'T{4| # 2Gh/:P[-z"Nw v J!!!"" * W &S1F
|!{Mz 0 5 U V R0*+drs yzL# t"u"v"w"#;#`#a### r/t q!x"6#~## 6 ,e> < z$ !!!#<#E#b#q#### ! 8W
l
Cq g r! ' ` X E on!!!h""n### u !"'"y"" s0
k "7##$ l A*f> =
j
!Qq ~ - F!!### n-5
1 1 R 4!c###$ 7 h}
= Lgf !k"=#F##, .Bz"- R?M t d{r,K% Q!!!
""r# | \'k@x R Y r " ##' l,1 q
g
3D`; P a ( h 3 s!""# " `X
!#. ;9]t+ m
@@F^ b ! x Th
Y r!# ( 3 ;
j
#zp!o!!!i""o# a M>FRp. y!"'#f###$ S' x I
H5 s /$ v3~n v
J6Wf$ )#S#)$ V}
cyTD ^ !C!!!="z"" B I} .h ? !! "("# ' 6 d F 1"G"""h##, lA!aQ
) Y
B
Hk H >f 0IA y + !{!""#) >k @lu(O
o)l 9_ l [!v!@"""8#$$ tjx1 4 :Mt%d=+s !=!# c aGS
0M~ 5"
$& L m +q >
! k
{
4#"rt !!"## o?g
uozB[ G!!"]# 6B? f] 2 0##7 y
Y
g
$
9
K
R
t
E R]b%|0 & F u . }"# o.
S`
Qq2p S " f6 P # ~ >l 2
>7 ;!!"d## u "
7
}r5!!!#$+ Uh6|gK ~
> hS [ # !!l"">## ]i
,
>
$ 8 )!tUL C"
Mg "G##? 6T.%P4S& Y
?,56s`z 'Lj&6&E< o \ R!! ""# $& wa
u et-EJZht !!T"e"$ /#Y:N z /7 f l 7!!"". C{V@
B T
wJ|[j
+p { @ +!!K#s#& } M D
)
]J=.bWAs/ , u d! +3Z ] O ! Z s 1!"#. s !"of 5
} (dgUVK+ADy ]
#@##[ XWdiv9 z
)
k
/ 7
"39UNgj?l/h4GY&| ; S L &!!+#U#&
m7s?^ r
G o = 8Ea ; % 4 J !!Y#) % j $y-
b +*39o i """
#C##$1 j& 1
' W @
e
h
!'i14j b ) t!". dw2j
2o*YX)n@Yh< Q ! # ; 5
/!#
P8)a& @!"## a@ P E1Pi !" s
Y
U ; 5 a ""#5 %Y:x,
6 _
DJ
'_<L L # "# <57F
@GNc V !4 = ~^#n
Q T w =
oAVTo#8 / !#!! "#G 9 u~F p
-
Z
x
f { (
n
-@Lr)AZv $ u ? U "-"n"""##+$ y U cil ( G y## `b !#
s! H
Z Noa h P" $ZyFv!^"" ) 4 T q= ; {xK " p!!!s"5#p#
_ k
"" ),g4<
L q E!P!!j"## _:+s
?wO ` !# w R:
=q-4 "## A |} &xSVeC E B!!!<"F" #(#g##! b j`kNP
nGr/@ ^ Z!z!?" $$ K 5 X J
Q
s
e{1 t |"0$ } R!
O :! T\({ Z "$ A y S
4s5I_y.i5 6 *!! *Wcn TX *#T#& r> i
1<)27W8Xg : ! : 4
h% : ""!#& wE o Y
w
e z ?K" 7 K T ""###*$ a` x# S +~
K YED!!]"r""4#" @ v _{r UN ] _ D !Y!!;">"E" J 4[W R
d4xhz Y N !{" D X
0S(7! "## I R ~
3wg\ X!!\"3#w# C D JMN?@AG /i6R V!W!## E KOBH
j7}^k @ "")"##%$! L'&C
B (8~#UWl @ #" K IG_i 2"H""###&$ F Y e h A G *""i#( cP fDk
.7v
9) X o o !!( K C
y$? JXm z '"
Z C
I[lw. i "$" mDB
B i , 7 |!##6 M(F\bH
R
*
)9V I!g1PE A k P !"""# AuL J )
! pi`j! \!!I""# ?l} mP Om + !!9#l# Gv?E 4^` = w!3"""#'$" mT
u*H
: m !A""I###$ $% q u
4 5
;N>,t = H !>!b!+"" 28
lq 3& G P 2pyB
4u&eW Z 5 f B ##$ Ek
.
2i j#$ ZPb 0
gEN7* p W c p !!6" d Q,w.
_w: !$## H l1/ & 2 l dT [
8X9 Y C $P ?
L
" a p
5X KAWb N8TzT]KY5 { ` O { !"# 0 ;K ~(nn
~ a !#7 M n gd<x
D l
|
6A$uzu@T9> , S e r !!!#l ,BL69eY\ry! 1
m
Yz\R!#%|En s 2PaQ
<u~nx r u z } C 8 !"" `# ( s [ K D
vpB} I
x p@(NJ\ xC(/| H!"%"#^##$ <hz_
H.8n)\ > q #1 ReI
5
CO!<m {[5 $ D j !
"""% 7'7 '
] c
'eIJq- # ] '
wL ^j N""# KIE< d,5 > I 8 T!Z"1##& CnCR@
2 go1C 3 N }!#!$> @ZGcl S
L
FW7:Fu !@Gx3 ' B G G Q v y 0 \h2 ~ E
^
+YPB"c",1BI % Y !"\ Ez)V< C.CO[ c U
h
>
& t
%
+
:
N
*0iG_9B\:Q&Wklfe~ ) l ^ M!8"e N8\ [0IU]
$
I
+ 8 a 2
S
u
HS)N^}#Jz "(^hkN)2QF , / / ~""""#C ]py / jH
"
Uj
a~
IaPkq
!]!!J"V"N## K^ ]
d
x
rjqe d k!"# v$M }v T "#S f B*T , / 4
a
; G h
`
Raq$R/;R^,<.[nv3'3Q" { !""$ g.7Pn9 # Q/E~
c
y !:# 7 W V
Bn !!!g"m#' @myQ= |
i P
Jp e
, Q " 0 [?&j_: a X ' 2 ""## SgL
w G x!!&#e#$.$ Hm -g5 ] > !!0"""($' ?kw@F
3
L$ 8x !
#
8
y~\a$s !!/##
q !!"# g
TK !B""# $? v5$OUX9 % X v>+ID& %sDz; e n [ !6!S"d""J##$ $g r V2Yhu L e y
4
C
j
. | ( 6
!2\ITM<c>U
.-3a*% + : N K \ %!c!!?#; i icv
%0]
& V ?
an)h0(m?i n $ I !""X# 7`?
D0OT 4 ` .!?!"S X4}"E ,
5 P S v '
<
,nqCUI?S Y&nu #> !"!!","m"""# GF 3p< : O! ^j|9
%m<dq,? 3 ! $& Q @
P
r
3r ^-4 0 )!9! 9 qmd y
>;1Vg
6 9 ""# ? 3Z*^z
J XM X C :"D"q"% H Q C
W
5v'f][ !"v#$$& b% "uT8hV ( X W g n n !( JE[
vOh j h F|l>
tN3] < !"H#k## $ 1
-
k 13 a!& Y+ - /
^86% B 1 k !#_ /
f:c}w5X
0
K
` l }
y@QtJVa{DmOM7;t8\}+ w q _ y B
d q
!!!"#( ;Q_' H r 4
BN -7mA|'4= # p "" \ JH&Q &
+ S!# $Y 7?[U/ TB Z } #
D *
1
]
/Vh*F6Ot[!(MPky '+j?FeH . F X ] 7""; e \x
\
: w
`u}.:Q]+;mu&P !U""M###$ P-xb< {
V h U
Io f"& R/K
v fFKi \ 1 !/""#%#$-$0 W$ W
=x%[` r$S d !".##F
b
3X x
- O {
SmL(H /-%` " m 3 -!!"""" ;q
2
+ 2 8!# ]$ c =~0S\7 ' V 8 W m p"""u#' 0
IZ 027 A g ; !!7 eG|
AW"
/
C k \
Ux)UZClLx*:s| E A " [V z
U
9\q [ #L#$) a2W
p
<K},6 1 ,! """t# ^_`DEFU
YkrZ D " :z qGACNNNNNNGTC,CCAGGCCTGG,GATCCGGATC,CCWGGTACCWGG,CCANNNNNTGG,CCAGGNNNTGG,CCANNNCCTGG,CCAGGNCCTGG,GCACNNNAACGTTNNNGTGC,CACNNNGTG,CCNNNNNNNGG,AACNAACRYGTGCGTGC,GCACGCACRYGTTNGTT,GCACNAACRYGTTNGTGC,AACGCACRYGTGCGTT,GACNNNNNGTC,GAANNNNNNNTTGG,CCAANNNNNNNTTC,CACNNNNGTG,AACACNNNNGTGC,GCACNNNNGTGTT,GCANNNNNNTGC,GAACNNNNNNTCC,GGANNNNNNGTTC,CAGNNNCTG,CCAGGNNCTG,CAGNNCCTGG,CCWGGGCCC,GGGCCCWGG,CCWGGGCCCWGG,AACNNNNNNGTGCACNNNNNNGTT,GACNNNGTC,GAANNNNTTC,CCWGGTACC,GGTACCWGG,CCWGGWCCWGG,ACNNNNGTAYC,GRTACNNNNGT,CCWGGYRCCWGG,GAAGNNNNNNTAC,GTANNNNNNCTTC,AACGGCNNNGTGC,GCACNNNGCCGTT,GCACGGCNNNGTT,AACNNNGCCGTGC,CGANNNNNNTGC,GCANNNNNNTCG,CGATCNNNNTGC,GCANNNNGATCG,TGANNNNNNTCA,TGATCNNNNTCA,TGANNNNGATCA,TGATCNNGATCA,AACCTGCNNGTGC,GCACNNGCAGGTT,GCACCTGCNNGTT,AACNNGCAGGTGC,GCCNNNNNGGC,AACNNNNNNGTGCMC,GKGCACNNNNNNGTT,CCWGGNCCWGG,AACTGGGNNGTGC,GCACNNCCCAGTT,GCACTGGGNNGTT,AACNNCCCAGTGC,GACNNNNGTC,GAGNNNNNCTC,CCWGGTCTC,GAGACCWGG,GATNNNNATC,GATCNNNATC,GATNNNGATC,GATCNNGATC,CCWGGCGCCWGG,ACNNNNNCTCC,GGAGNNNNNGT,AACNNNNNNGTGCAG,CTGCACNNNNNNGTT,GATCATGATC,CCWGGNNCCWGG,AACTGGNNNGTGC,GCACNNNCCAGTT,GCACTGGNNNGTT,AACNNNCCAGTGC,AACNAACCGGTGCGTGC,GCACGCACCGGTTNGTT,GCACNAACCGGTTNGTGC,AACGCACCGGTGCGTT,GCANNNNNTGC,GCACNNNNNNGTTCGAACNNNNNNGTGC,CCTNNNNNAGG,GCNNNNNNNGC,CCANNNNNNTGG,CCAGGNNCCTGG,CCTGGCCAGG,CAANNNNNGTGG,CCACNNNNNTTG,TTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAA,GCACNNNNNNGTTTAAACNNNNNNGTGC,CCAGGNCCY,RGGNCCTGG,CCWGGGNCCTGG,CCAGGNCCCWGG,AAGNNNNNCTT,GAYNNNNNVTC,GABNNNNNRTC,GAYNNNNNRTC,GATCNNNNVTC,GABNNNNGATC,GAYNNNNGATC,GATCNNNNRTC,GATCNNNGATC,GTYAACNNNNNNGTGC,GCACNNNNNNGTTRAC,GCACNNNNNNGTTAACNNNNNNGTGC,GATCNGATC,TAACTATAACGGTCCTAAGGTAGCGA,TCGCTACCTTAGGACCGTTATAGTTA,CTCTCTTAAGGTAGC,GCTACCTTAAGAGAG,TAGGGATAACAGGGTAAT,ATTACCCTGTTATCCCTA,GTTAACNNNNNNGTGC,GCACNNNNNNGTTAAC,AACNAACGCGTGCGTGC,GCACGCACGCGTTNGTT,GCACNAACGCGTTNGTGC,AACGCACGCGTGCGTT,TCCAACNNNNNNGTGC,GCACNNNNNNGTTGGA,CAYNNNNRTG,AACAYNNNNGTGC,GCACNNNNRTGTT,GTTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAAC,CCWGGCGCC,GGCGCCWGG,CCWGGNNCC,GGNNCCWGG,GATCGCGATC,AACNAACATGTGCGTGC,GCACGCACATGTTNGTT,GCACNAACATGTTNGTGC,AACGCACATGTGCGTT,TGGCAAACAGCTATTATGGGTATTATGGGT,ACCCATAATACCCATAATAGCTGTTTGCCA,ATCTATGTCGGGTGCGGAGAAAGAGGTAATGAAATGG,CCATTTCATTACCTCTTTCTCCGCACCCGACATAGAT,AACNNGCACGTGCNNGTT,GAACNNNNNCTC,GAGNNNNNGTTC,CCAGGWCCY,RGGWCCTGG,CCWGGGWCCTGG,CCAGGWCCCWGG,CCAGGWCCTGG,TTATAACNNNNNNGTGC,GCACNNNNNNGTTATAA,GCACNNNNNNGTTATAACNNNNNNGTGC,GAACNNNNNNTAC,GTANNNNNNGTTC,GGCCNNNNNGGCC,AACNNACTAGTGC,GCACTAGTNNGTT,AACTAGTNNGTGC,GCACNNACTAGTT,AACNAACTAGTGCGTGC,GCACGCACTAGTTNGTT,GCACNAACTAGTTNGTGC,AACGCACTAGTGCGTT,AACNNNAACGTGCNNGTGC,GCACNNGCACGTTNNNGTT,GCACCCANNNGTT,AACNNNTGGGTGC,TTAACNNNNNNGTGC,GCACNNNNNNGTTAA,CACNNNNNNTCC,GGANNNNNNGTG,CACNNNNGATCC,GGATCNNNNGTG,GATCTAGATC,CCANNNNNNNNNTGG,CCAGGCCT,AGGCCTGG,CCTCGAGG,GATCCGGA,TCCGGATC,GGCGCGCC,GCGATCGC,CCWGGWCC,GGWCCWGG,GATCGATC,GATCATGA,TCATGATC,GCGGCCGC,CCTGGCCR,YGGCCAGG,CCWGGNCC,GGNCCWGG,GGCCGGCC,RTGCGCAY,GATCNNGA,TCNNGATC,CGCGCGCG,CCTGGCCA,TGGCCAGG,CGCCGGCG,GTTTAAAC,GATCGCGA,TCGCGATC,TTAATTAA,VCTCGAGB,CCTGCAGG,CRCCGGYG,CGTCGACG,ATTTAAAT,GCCCGGGC,GATCTAGA,TCTAGATC,CACCTGC,GCAGGTG,CCTNAGG,GATCGAT,ATCGATC,CCTCAGC,GCTGAGG,GCTNAGC,CCTNAGC,GCTNAGG,GCTCTTC,GAAGAGC,GGTNACC,CGGWCCG,ACCWGGT,RGGNCCY,CCTGGAG,CTCCAGG,GGTGATC,GATCACC,GATCNGA,TCNGATC,GGGWCCC,GAAGATC,GATCTTC,CCCWGGG,TCCNGGA,TCCWGGA,RGGWCCY,TTATAA,AGGCCT,GACGTC,GTMKAC,TCCGGA,TGCGCA,ACCTGC,GCAGGT,GGTACC,AGTACT,GGYRCC,CCGCTC,GAGCGG,AACGTT,YGGCCR,RAATTY,CTGAAG,CTTCAG,CACGTG,GRCGYC,AGCGCT,CTTAAG,ACRYGT,ACCGGT,ACTAGT,CACGTC,GACGTG,GWGCWC,GTGCAC,CYCGRG,GGGCCC,ATTAAT,CCTAGG,TTCGAA,GCTAGC,GKGCMC,TGGCCA,GGATCC,GRGCYC,ATCGAT,CACGAG,CTCGTG,GGCGCC,GAAGAC,GTCTTC,GCATGC,GTATCC,GGATAC,TGATCA,ACTGGG,CCCAGT,CTRYAG,RGCGCY,AGATCT,GGNNCC,CTGGAG,CTCCAG,CTTGAG,CTCAAG,CGATCG,GGTCTC,GAGACC,YACGTR,CCNNGG,GAATGC,GCATTC,WCCGGW,GCAATG,CATTGC,RCCGGY,GCGCGC,GAGGAG,CTCCTC,CGGCCG,CCCAGC,GCTGGG,GTGCAG,CTGCAC,CGRYCG,CGTACG,CGTCTC,GAGACG,CCATGG,TCGCGA,GDGCHC,TGTACA,TCATGA,ACATGT,GTATAC,CCWWGG,CTCTTC,GAAGAG,GCNNGC,CCRYGG,GTTAAC,RCATGY,TACGTA,RGATCY,GCGATG,CATCGC,GCAGTG,CACTGC,CCCGGG,CCGCGG,TTTAAA,GGCGGA,TCCGCC,GAGCTC,GATATC,CTGRAG,CTYCAG,CAGCAG,CTGCTG,GAATTC,ATGCAT,CATATG,GTYRAC,AAGCTT,GTNNAC,TCNNGA,CAATTG,ACGCGT,TCCRAC,GTYGGA,GCCGGC,CMGCKG,GCCGAG,CTCGGC,CTCGAG,CTGCAG,CAGCTG,GTCGAC,CTYRAG,AATATT,GATCGA,TCGATC,GACCGA,CACCCA,TCGGTC,TGGGTG,WGTACW,TARCCA,TGGYTA,TCTAGA,GGATC,GATCC,CCWGG,GTCTC,GAGAC,GCWGC,GGNCC,CCSGG,GGTGA,TCACC,GGWCC,GCAGC,GCTGC,CCATC,GATGG,ACGGC,GCCGT,GCNGC,CCNGG,GCATC,GATGC,ACTGG,CCAGT,GGATG,CATCC,CTCAG,CTGAG,GGGAC,GTCCC,ACNGT,CTNAG,CAGTG,CACTG,GACGC,GCGTC,CCCGC,GCGGG,GANTC,CGWCG,TCNGA,CCTTC,GAAGG,GTNAC,GAAGA,TCTTC,GAGTC,GACTC,GTSAC,GAWTC,GCSGC,CASTG,ATGAA,TTCAT,ACGGA,TCCGT,CGCG,CCGC,GCGG,GTAC,AGCT,GCGC,CTAG,GATC,GGCC,CCGG,CATG,RGCY,YATR,ACGT,TGCA,AATT,CCTC,GAGG,TTAA,ASST,TCGA = n
" . < p %
) E ! 1 %
Z
J > Y f * i 5 L _ " Y ' ) y = n
" . < p %
) E ! 1 %
Z
J > Y f * i 5 L _ " Y ' ) y R v < <"P{
M#M#DPZAH r {
UVZ[yrs' ] m ! "M#
" w & !d
"
[]!
["z_# C
9 yd 4 ^ R5 = !! Z v;^ /G[dk|
%
= I s
jsRW8: ENT 9BGK R 5
*v
E "P r
(5E} "
(5Ed "
(5E " (}(5Ed (5E (( x ' ! ! > ! ` u !^ } x L }Y " ` ! ` i!\ !\ ]4}
Wz = ^ % ( qE [L# = u Q
p2 ! # N i ""M
's * ~ b"*
h 5 #! 9 z Njl<Y +# "&" h
0 '* CA< 8c| K!X? !6 . 6
UL }&#"F6
a"9 l +#Q K|LZ'* V G
& 'jt!K `EQCAMa < $ r ]@8i\'c| * K!r,W"MQ
r BV=K| 's * [ ~ > b" ^ #q;
l _
5 [\?IW_r &
`
VK Uki,rL`r !#L#M#
" b?7* c p !!!Am ($v4
?Pszv x ! E
<
9\ B
' [H* M R_ < "C
-Z#i#b9A 6
LZ . a"^Qi #]4 FDt
!
i
9 \ x
1T(8WPT&E=GP*#$2w)lO!%IO$N~v 9 x J w $!"? &
Vir !#>Q rb;K|!y # !" ! UL?VR 3# &l
Z0
^h #, a
"
P S <
n "! h * Q6h
Fp 22 * !$ mpmb
l9`^A z !#Pw)Pv x f ?U B Z
/VFR;R^P<k'Q ] "^q @{J Ef
R;R<' ql;X
" X A
Gj J#"Ns o7 "] &!Q b;K d| !R /L}\x Xx - {
SL `} -` U"M##D
-Z#W!D+ . 3 +
F R g u
&
;
_
+`mp#BQT~R Xm-Z2z !!Y<
:' LV> a > e|W/
K
` k
xJUVaClLM7:s\|_ A "" E m
% )W} #A#$T u*H
: m I##$ $V > + . 3
F g
_
`#Q~-Z2z W!D +
R u &
;
+mpBTR Xm !!K "{ ?Q +q
"r "[ I %
*>AW!8dD[x + . 3 | +
F R g u
&
)
;
_
+.X`[mp#BNQT
~OR 1X`m
-PZi2>dG z t - | 7 !!!" \ex |XWx /
K
- ` k {
xSL J`UV}aCl -LM7`:s\| _ A "U"M##j
#
w !" \*
E] ~ !UgK [ !># u [) o
P } !F
4" !] $ I !X#r Z
W L m 6
%P > k
{
#tS S R!!"## $ ] $ I !!X# v g !x f
D/% E
1 n0M A"
C \
U)Zx* E " o
W@ SyS4 N z t c j ( "
C!m[C $ D N
"#uP J H $~S7 ' V m :)(fSQ ~
X M !###q ]4E# W f t( S
X ! c j ">A8d[x |
)
.X[N
O1`
Pi>dG t - | 7 !" gv !, h "# 2""&$ 1$ @ZGcl S
L
FW7:Fu !@Gx3 ' B G G Q v y 7?[U/ TB Z } #
D *
1
]
/Vh*F6Ot[!(MPky '+j?FeH . F X ] 7"" J
A;8d'[mx
m
o
5XW[
@`1 `PSSy=S 4 t R | N 7 z !"| z "g 0+
H
rS\.a<fS=(4# i $DZ[;BHg-Nk1 b T
7 = % ` s
M
^8AXEGA9!j"]AwM(d2 } ( + $ x L!GF =it' `R ( X A
(/9Gjsc<L * =e}kH * !u!- Z Y=
" : v {
^
:' OMk&Fm ?Q5=Z ! T b 9 !,## /
f:c}w5X
0
K
` l }
y@QtJVa{DmOM7;t8\}+ w q _ y B
d q
!!!"#0 [?&j_: a X ' 2 ""##- Z g Y`X=HW0k+Usz
" : v {
*
2
A
$ H ^ i 8
#:SZr';Q OlMS\.ak{&0<fFOS#-Gmt ?Q15=b4=cp(4ZM[ # ! . L T ] b w D I 9 i '!_!!!!",#V##$w R_S:+gs0L:
w
[?&G=?jq-_w: O a ` 4 X ' 2 !x!!"""#&#e####$.$6B? f] 2 0##<57F
@GNc V ! u !"'"y"" 0
IZ 027 A g ; !!n:a$K_ t
\ L p E
>_
9dW J < g!S +~
K YED!!]"r""4#g z /6B0#+Yk: ?
H N z M
rfS\.a<f/Se=H| ]l(4# r 2 7 f l i 7!!""0###$ '"
Z C
I[lw. i "$" - !00FivePrimePhosphorylatedFivePrimePhosphorylated
3ac69535-eb75-47ee-953f-b330626baaf4
Synthetic
0
pAC-94N
1
Contains crtI, crtY, and crtI genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and produces beta-carotene in E. coli when complemented with a gene encoding geranylgeranyl diphosphate synthase.
2021.5.13
2021.5.13
UNA
unspecified
Y
????? d HO d E Y? P 3
)